This is a list of necessary items and equipment you need to perform a NADMED assay:

The current NAD measuring kits are not accurate enough to provide results that can guide e.g. NAD boosters dosage in proper order. See more “Groundbreaking technology”.

Whole blood (human) 

– Blood should be collected into the smallest EDTA vacutainer (2 ml)
-IMPORTANT! If possible, the blood sample should be divided into 150 – 200 μL aliquots in separate test tubes, e.g. Eppendorf tube
-Samples should be frozen at -20 °C within 4-6h after withdrawal for several days. Samples should be stored for longer time in -80 °C
IMPORTANT! Keep consistent time between withdrawal and freezing for all samples

Whole blood (animal)

-Blood should be collected into the EDTA tubes and divided into 150 – 200 μL aliquots in separate test tubes, e.g. Eppendorf tube
-Samples should be frozen at -20°C as soon as possible after withdrawal for several days.
-Samples should be stored for longer time in -80°C
IMPORTANT! Keep consistent time between withdrawal and freezing for all samples
Read more

In -20°C freezer with consistent temperature.

40 samples measured in duplicates, each metabolite (e.g. NAD+ and NADH) are measured on separate 96 well plates.

NADMED method can utilize whole blood, tissues and cultured cell (human or animal). Most NADs are found in cells – plasma and serum contain very low levels of NADs and are not suitable for NADMED technology.

For more details, please contact us

We recommend using 150 – 200μL/sample of whole blood, approx 20 mg/sample of tissue, and 0.5 – 1M/sample of cultured cells. For more details, please contact us.

Instruction can be found on our website in section “documents”.

No, the kit is for one time use. We do not recommend freezing / thawing the kit components.

• Blood: NAD levels are normalized per volume (final concentration is in μM)
• Tissues/cells: NAD levels are normalized per sample weight or protein amount

Yes, but they often require specialized equipment and have low throughput. NADMED stands out because it is:

• Simple
• Scalable
• Accurate
• Suitable for many sample types, including     blood

1. Simple: No complex instruments required
2. Robust: Uses a validated enzymatic, colorimetric detection method
3. Ouantitative: Results shown in clear concentration units
4. Versatile: Works with many sample types
5. Standardized: Suitable for diagnostic use
6. Scalable: Can analyze many samples at once

ln diagnostics, NADs and glutathione are measured from whole blood.

• NAD+ indicates vitamin B3 status
• NADP+, NADPH, and glutathione reflect oxidative stress

Results should always be interpreted in the context of your health, lifestyle, and medical history.

Metabolism affects everything your body does – from how you use energy to how you respond to stress.

The NADtest gives you a snapshot of your metabolic status and shows whether you may benefit from vitamin B3 supplementation. lf you’re already supplementing, the test helps you see how well it’s working.

Vitamin B3 deficiency can happen for two main reasons:

1. Not getting enough vitamin B3 from food – this can cause pellagra, a serious but now very rare condition.
2. lncreased demand for vitamin B3 due to stress, illness, or lifestyle factors – even with a normal diet.

Stress factors include:

• Metabolic, cardiovascular, kidney, or genetic conditions
• Digestive issues and malabsorption
• Certain medications
• Chronic alcohol use
• lnfections and immune responses

When the body uses more NAD+, levels may drop, leading to symptoms such as fatigue, skin issues, or digestive problems.

Vitamin B3 (niacin) becomes metabolically active only after your cells convert it into NAD+.

This activated form is what your body actually uses for energy production and many essential cell processes.

NAD+ is vital for life. lt helps your cells:

• Convert food into energy (ATP)
• Respond to stress
• Repair damage
• Regulate metabolism

Your body constantly uses and replenishes NAD+ to keep you functioning.

Yes. There are four forms of NAD, often called NADs:

• NAD+ and NADP+ (oxidized forms)
• NADH and NADPH (reduced forms)

They work as cellular “energy shuttles,” carrying electrons where they’re needed.

No. Different tissues need different amounts depending on their functions, so NAD levels vary across the body.

NADPH is particularly important. lt:

• Helps cells grow
• Protects them from oxidative stress
• Supports the production of lipids and DNA building blocks
• Keeps the antioxidant glutathione in its active form

Glutathione is ane of the body’s most important antioxidants. lt protects cells from oxidative stress – the “wear and tear” caused by normal metabolism.
Glutathione exists in two forms:

• Reduced glutathione (GSH) – active
• Oxidized glutathione (GSSG) – inactive

NADPH helps recycle oxidized glutathione (GSSG) back into its active form (GSH). This process is essential for protecting your cells from oxidative damage.

lt helps reveal how well your body handles oxidative stress.

Higher levels of reduced glutathione generally indicate better antioxidant capacity.

Common causes include:

• Not getting enough amino acids needed for glutathione production
• Acute stress, like infections
• Chronic stress or long-term health conditions

Think of “reduced” mol ecu Ies as charged with energy and “oxidized” mol ecu Ies as used up.

NADs and glutathione constantly switch between theseforms to support metabolism and protect the body.

We compare your results with reference levels established from 300 healthy individuals. This helps determine whether your levels are low, normal, or high. 

Both matter, but levels come first.
For example:

• If NAD+ is low, many NAD-dependent processes slow down.
• lf NAD+ is normal but NADH is high, it may inhibit some metabolic reactions.

Low NAD+ always has a cause – and it’s not aging. lt may indicate:

• Poor diet or malabsorption
• Chronic or developing health conditions
• Higher NAD+ demand due to inflammation or disease

Blood aisa responds to tissue needs, so low blood NAD+ can reflect increased use in affected tissues.

ln blood, NAD+ levels stay stable from ages 18 to 70 in healthy individuals.

ln tissues, levels may decline slowly after age 70 – but the change is not dramatic. Exercise helps slow or prevent NAD+ decline in muscle tissue.

Because more isn’t always better.
High-dose vitamin B3:

• 1s fully absorbed
• Raises NAD+ but also raises other metabolites
• Can increase breakdown products that may cause inflammation

Supplementation should only be used when deficiency is confirmed.

For individuals with very low NAD+ levels, supplementation may help restore energy and improve the body’s ability to respond to medical treatments. Research has shown potential benefits in conditions like mitochondrial disorders, where patients experience increased strength and symptom relief, as well as in neurodegenerative diseases like Parkinson’s, where NAD+ may support neurological function.

While the potential benefits are vast, more human studies are necessaryto confirm the effectiveness of NAD supplementation for different conditions. Current research suggests that supplementing with NAD precursors is mostly beneficia l when NAD levels are low. Because of this, it’s best to monitor NAD levels under medical supervision before starting supplementation. Over-supplementation can lead to imbalances, as excess NAD+ may be broken down into byproducts that could have negative effects. ln summa ry, the aim is to maintain balanced NAD levels, not to artificially boost them beyond the body’s needs.

The most well-researched method for boosting NAD+ involves oral supplementation with B3 derivatives like niacin, nicotinamide riboside (NR), or nicotinamide mononucleotide (NMN). Niacin is the fastest-acting option but can cause temporary skin flushing. NR and NMN provide a slower increase in NAD+ with fewer side effects.

Choosing high-quality, tested supplements is essential, as studies have found inconsistencies in some NMN products. While IV infusions of NAD+ have gained attention, research supporting their effectiveness remains limited. ln most cases, oral supplementation is the most reliable and accessible option.

For the best results, NAD+ levels should be monitored under medical supervision to avoid unnecessary supplementation or potential side effects.

Research confirms that niacin, NR, and NMN can increase NAD+ levels, but their effectiveness depends on an individual’s baseline levels and overall health status. Simply raising NAD+ is not always beneficial. What matters most is maintaining the right balance between NAD+ and NADH.

This ratio plays a critical role in mitochondrial function and metabolic efficiency, making it important to focus on equilibrium rather than excessive supplementation.

Supplements are not the only way to support NAD+ levels. Regular exercise, especially highintensity training, stimulates natural production. Adiet rich in vitamin B3, found in foods like fish, mushrooms, nuts, seeds, and legumes, can also help. lntermittent fasting activates cellular repair processes that enhance NAD+ metabolism, while quality sleep supports its natural regulation. Reducing alcohol intake and managing stress further prevent unnecessary depletion.

A balanced lifestyle, rather than supplementation alone, provides the most sustainable way to maintain optimal NAD+ levels.